Figure 2
In vivo STED microscopy of filamentous actin marked with Lifeact fused to the far-red emitting fluorescent protein mNeptune2. A dendritic branch at 6 µm depth of the visual cortex is recorded at 560 nm excitation in the confocal mode (a) and by an additional laser at 732 nm for stimulated emission depletion (STED) in superresolution mode (b). The correction collar of the objective was used to optimize the resolution in the tissue8. (c) Image section of the marked area in (b) of 17 time points (t) recorded within 55 min, each with a maximum intensity projection of 4 z-slices of raw data 500 nm apart. For the whole segment see the Supplementary Movie S1.
