Figure 4
Dendritic morphology at high and low expression levels of actin binding fusion proteins. Confocal scans of a brain slice at the region indicated by a star in the boxed overview image (a,a’). (a) Lifeact-mNeptune2 expression in L5 in the periphery of the fluorescent area shows plenty of dendritic spines which are abolished in the central region (a’). (a’) Bright accumulation of fluorescent fusion proteins which are not present at lower expression levels in the periphery of the labelling (a). (b) Actin-Chromobody-mNeptune2 expression in L5 highlights spines and dendrites; rare overexpressing dendrites. (b’) No spines are visible at high expression. (c) Actin labelling of spines and a cell body at low expression compared to thick bundles of actin in dendritic shafts without spines at high expression levels of Lifeact-tagRFP657 (c’) within the same brain slice. Note the increase in brightness between the centre and the periphery of the labelling.
