Figure 1
From: Acid Sphingomyelinase Inhibition Prevents Development of Sepsis Sequelae in the Murine Liver
Increased SMPD1 activity is highly associated with liver dysfunction in polymicrobial sepsis. Polymicrobial sepsis was induced in smpd1+/+ and smpd1+/− mice by peritoneal contamination and infection to determine (A) serum SMPD1 activity (Fig. 1A; n = 4 smpd1+/+, n = 8 smpd1+/−), (B) liver glutathion (GSH) levels (Fig. 1B; n = 4 smpd1+/+, n = 4 smpd1+/−), (C) γ-GT, (D) serum total bilirubin (Fig. 1C,D; n = 4 smpd1+/+, n = 8 smpd1+/−) as well as (E) hepatic transcriptional expression of Mrp2 (Fig. 1E; n = 4 smpd1+/+, n = 8 smpd1+/−) at three different time points (24 hours, 3 and 28 days). #p < 0.05; ##p < 0.01 vs. corresponding smpd1+/+ control; *p < 0.05; **p < 0.01 vs. baseline (MWU-test). Transcriptional expression is normalized to reference gene (Actb) and shown as log2 fold changes. Cut off values were set at ± 1, representing a variation of biological significance (dotted lines).
