Figure 5
From: Acid Sphingomyelinase Inhibition Prevents Development of Sepsis Sequelae in the Murine Liver
Preventative treatment strategy as well as therapeutic treatment diminishes hepatic inflammatory response during polymicrobial sepsis. Hepatic gene expression of (A) f4/80, (B) Ccl2/mcp1, (C) Il1b and (D) Tnfa was determined at three different time points following polymicrobial sepsis (24 hours, 3 and 28 days; n = 4 smpd1+/+, n = 8 p-smpd1+/+, t-smpd1+/+) to evaluate the impact of SMPD1 on hepatic inflammation following either preventative (p-smpd1+/+) or therapeutic (t-smpd1+/+) treatment with desipramine during sepsis. Transcriptional expression is normalized to reference gene (Actb) and shown as log2 fold changes. Cut off values were set at ± 1, representing a variation of biological significance (dotted lines). #p < 0.05; ##p < 0.01 vs. corresponding smpd1+/+ control; *p < 0.05; **p < 0.01 vs. baseline (MWU-test).
