Figure 2

GRB2 associates with MPZL1 in a phosphotyrosine, SH2 domain-dependent manner. (a) HEK293T were transiently transfected with FLAG-GFP or GRB2 and lysates were used to perform an AP followed by Western blot analysis. (b) HEK293T were transiently transfected with FLAG-GFP or GRB2 (WT or point mutants), treated with PV and utilized as in (a).