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Figure 1

From: Cold atmospheric helium plasma causes synergistic enhancement in cell death with hyperthermia and an additive enhancement with radiation

Figure 1

He-CAP and HT induced synergistic effects. (A) Annexin-V FITC/PI assay carried at 6 h after combined treatment with He-CAP and HT (n = 4). (B) Representative flow cytometry histograms of Annexin V-FITC/PI staining are shown. (C) Cells were harvested 6 h after combined treatment and subjected to DNA fragmentation assay (n = 3). (D) He-CAP and HT induced morphological features were detected by Giemsa staining. Images were taken at ×400 magnification under microscope. One representative photomicrograph is shown here, arrow head shows apoptotic cells. All the data are presented as mean ± SD. ***p < 0.005, vs. HT alone.

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