Figure 1

Study design. (a) The study population comprised 5 groups of pigs (n = 5/group). Groups 1 to 5 were used for the histopathological characterization of myocardial tissue changes during the first week after ischemia/reperfusion (I/R). CMR (cardiac magnetic resonance) scans included cine steady-state free-precession (SSFP) sequences to provide high quality anatomical references and functional information. CMR scans were performed at all follow-up stages until sacrifice, so that animals sacrificed at day 7 underwent baseline, 120 min, 24 h, day 4, and day 7 CMR. CMR and myocardial water content data from animals in groups 1 to 5 have been reported previously¹⁸. (b) For proteomic analysis, remote and ischemic tissue samples from these animals were processed for protein extraction, tryptic digestion, multiplexed stable isotope labeling, and fractionation followed by nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) and systems biology analysis. Graphical elements in panel b were adapted from the Servier Medical Art Powerpoint image bank. Servier Medical Art by Servier (http://www.servier.com/Powerpoint-image-bank) is licensed under a Creative Commons Attribution 3.0 Unported License (https://creativecommons.org/licenses/by/3.0/).