Figure 5

Quantitative proteomics time course analysis of early reperfusion after acute infarction in the remote myocardium. Quantitative proteomics screening approach results are presented as in Fig. 3. Significantly altered functional categories were classified into five clusters (a–e): Cluster a, showing a transient increase at 120 min or 24 h; Cluster b, decreasing at 120 min and 24 h and reverting to almost normal values at day 7; Cluster c, reaching maximum upregulation at day 4, Cluster d, increasing gradually over time (this cluster included ribosome and collagen categories for comparison of results with the infarcted area); and Cluster e, decreasing in the late phase of reperfusion, reaching minimal values at day 7. Panel (f) shows a schematic chart of changes in water content and protein clusters. The complete set of proteins changing in each category is listed in Supplementary Tables S5–S9. The proteomics results presented here are representative of three technical replicate experiments using five animal sample pools.