Figure 5
From: Time-resolved analysis of DNA-protein interactions in living cells by UV laser pulses
Potential use of a custom microfluidic device in L-ChIP. (a) First and (b) second set-up of microfluidic system used. (c) Proliferation curve by trypan blue of MDA-ERα-GFP irradiated at the indicate energy doses. (d) ChIP assays performed in cells irradiated at Epulse = 7 μJ and RR = 2 kHz for 8 passes showing TRAIL promoter region occupancy by H3K4me3. ChIP data obtained on immunoprecipitated fractions were normalized to input chromatin (IP/Input). Curves show the mean of at least two independent experiments with error bars indicating standard deviation.
