Figure 3
In vivo time evolution of [U-2H7, U-13C6]-D-glucose and its metabolic product [13C3]lactate in the mouse head. A 20° flip angle pulse was applied every 0.5 s on the carboxyl region of the spectrum while the flip angle was measured to be 1.4° on the substrate region. To improve the SNR every two spectra were added leading to actual time resolution of 1 s. Glucose C1 peaks appear at 96.8 ppm (C1-β) and 93 ppm (C1-α), lactate is observed at 183.5 ppm and can be identified 10.5 s after the infusion (bottom). The corresponding time course (inset) of the spectral data that was quantified using AMARES while fitting the lactate doublet to two separated peaks.
