Figure 5
In vivo 13C MRS in the mouse head following the injection of hyperpolarized [2,3,4,6,6-2H5, 3,4-13C2]-D-glucose. Spectra were acquired by applying 20° flip angle at the carboxyl resonances and −0.05° flip angle at the substrate resonances every 0.5 s. To improve the SNR every two spectra were added leading to effective time resolution of 1 s. Glucose peaks appear at 75.3 ppm (C3-β), 73.8 ppm (C3-α) and 70.7 ppm (C4-α and C4-β) and lactate is observed at 183.5 ppm. The corresponding time course (inset) of the spectral data was quantified using AMARES.
