Figure 3
Honokiol activates cardiac PPARγ signaling. (A and B) H9c2 cells were transiently co-transfected with ACO-PPRE, PPARγ luciferase reporter gene and Renilla luciferase expression vector. 24 h after transfection, cells were treated with Honokiol (0, 2.5 μM, 5 μM) for one day. Then, cells were lysed and examined for reporter gene expression. The experiment shown above is representative of three independent experiments. (C) Relative expression levels of PPARγ mRNA were determined by Reverse transcription-PCR. (D and E) Protein levels of PPARγ relative to GAPDH in cardiac tissue homogenate. (n = 3–5). (F and G) Relative expression levels of SOD2 and CD36 mRNA were determined by Reverse transcription-PCR. (H) Cardiac tissue homogenate was prepared and analyzed for lysine-acetylation using anti-acetyl lysine antibody. (I) Quantification of relative lysine-acetylation (n = 3–5). *p < 0.05 vs. Control group; **p < 0.01 vs. Control group; #p < 0.05 vs. Dox group; ##p < 0.01vs. Dox group. Values are expressed as mean ± SEM. Full-length images of blots and gels presented in supplementary information.
