Figure 2 | Scientific Reports

Figure 2

From: Refinement of ectopic protein expression through the GAL4/UAS system in Bombyx mori: application to behavioral and developmental studies

Figure 2

Improved visualization of projection pattern of PTTH neurons. (A,B) Fluorescent images of wandering larval brains under an epifluorescent microscope. mCD8GFP (A) or myrGFP (B) were expressed by PTTH-GAL4 strain. White arrowheads and yellow dotted circles indicate cell bodies and corpora allata (CA), respectively. (C) Quantification of GFP fluorescence intensities in the cell bodies and CA. (D,E,G,H) Confocal images of immunostained brains (D,E) and CA (G,H). White arrowheads and yellow arrows indicate cell bodies and neurites, respectively. Magenta signals outside PTTH neurons are background DsRed expression driven by selection marker (3xP3-DsRed). (F,I) Relative fluorescence intensities of GFP in the cell bodies (F) and CA (I). *P < 0.01, **P < 0.005, ***P < 0.0002, t-test. Bars, 500 μm (A) and 100 μm (E,H). Number of samples are indicated in the parentheses.

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