Figure 6

Blockade of PTTH neural transmission delays developmental timing. (A) Timing of V0 larval molting. *P < 0.00005, Chi-square test. (B) Developmental change in larval body weights. W indicates the day when majority of the larvae started wandering. (C) Confocal image of immunostaining in CA. (D,E) Body weights in day 2 pupae (D) and adult moths (E). (F) Pupal duration. (G) Survival rate during larva-to-pupa development. (H–J) 20E titer determined by ELISA in female larvae (H), male larvae (I), and pupae (J). Statistically different groups are shown in different characters (P < 0.05, Tukey-Kramer’s HSD test after ANOVA). N.S., not significant (P > 0.05). Bar, 100 μm. Number of samples are indicated in the parentheses.