Figure 3

Effects of pulmonary inflammation and hypoxia exposure on the MuRF-1 mRNA contents determined by RT-PCR (a) and the myogenin contents (b) determined by Western blot (c), in soleus muscle subjected to an overload surgery. (n = 5–6; Mean ± SEM); Hprt1 was used as a housekeeping gene. NC: Normoxia Control; NS: Normoxia + Surgery; NSI: Normoxia + Surgery + Inflammation; HS: Hypoxia + Surgery; HSI: Hypoxia + Surgery + Inflammation. †NS vs. NC, p < 0.05; *global effect of inflammation (NS + HS) vs. (NSI + HSI), p < 0.05. Blot image has been cropped to the area of interest. The full-length blot is presented in Supplementary Figure 3.