Figure 1
From: Enabling stop codon read-through translation in bacteria as a probe for amyloid aggregation

Amyloidogenic peptide tagging at the N-terminus of RF1 enables aggregation-dependent stop codons reading-through by E. coli ribosomes. (a) A bacterial ribosome assembled around a stop codon, showing the tRNAs bound at the P (peptidyl) and E (exit) sites and a RF1 molecule bound at the A (amino-acyl) site. The solvent-accessible N-terminus of RF1, where the WH1(Rn) peptides were attached is indicated. Structure rendered with PyMOL v.1.4 (https://www.pymol.org) on the PDB file 4v63. (b) A cartoon of the functional expansion in translation termination implemented in this report: bacterial ribosomes recognize or read-through amber stop codons depending on the solubility (left) or aggregation (right) of peptide-tagged RF1 (yellow).