Figure 3
Fluorescence lifetime of the FRET donor mTurquoise2 fused to different FRET acceptors. The phase lifetime of mTurquoise2 (mTq2) when paired with different acceptors is depicted. As a reference the lifetime of untagged mTurquoise2 is shown. The dots indicate individual cells and the error bars show 95% confidence intervals. The number of cells imaged is mTq2 n = 89, EGFP n = 26, Clover n = 18, mNeonGreen n = 14, SYFP2 n = 72, sREACh n = 27, mOrange n = 21, mOrange2 n = 27, mKO2 n = 17, mKOκ n = 17, TagRFP-T n = 20, mRuby2 n = 17, mScarlet-I n = 30, mCherry n = 24, mKate2 n = 22.
