Figure 7
FlIM-FRET of Gq activation biosensors equipped with novel FRET pairs. The fluorescence lifetime of mTurquoise2 was recorded from the biosensor for Gαq activation containing mNeonGreen, SYFP2 or sREACh as FRET acceptor. The phase lifetime was recorded before addition of (ant)agonist, 20–60 s after addition of 100 μM histamine and 20–60 s after addition of 10 μM pyrilamine. The changes in phase lifetime are shown in the graphs. The grey lines represent individual cells and the black graph represents the average of which the error bars indicate the 95% confidence intervals. The number of cells used for the graph is for mNeonGreen as FRET acceptor n = 17 (out of a total of 26 cells), for SYFP2 as FRET acceptor n = 7 (out of a total of 23 cells) and for sREACh as FRET acceptor n = 46 (out of a total of 60 cells).
