Table 1 Spectroscopic parameters of fluorescent proteins employed in this study as acceptor for mTurquoise2. The overlap integral was determined from spectra acquired in this study and extinction coefficients taken from literature. The Föster radius was calculated from J(λ) and quantum yield of the donor (QY _D ) = 0.93, n = 1.33 and κ ² = 2/3.

EGFP

488

55000²²

1.53

0.6²²

55

Clover

505

111000⁸⁶

2.7

0.76⁸⁶

60

mNeonGreen

505

116000⁶³

3.15

0.8⁶³

62

SYFP2

515

101000¹⁸

2.31

0.68¹⁸

59

sREACh

517

100000^70,87

2.53

—

59

mOrange

548

71000⁶⁵

2.19

0.69⁶⁵

58

mOrange2

549

58000⁴³

2.05

0.60⁴³

57

mKO2

551

63800⁸⁸

1.44

0.57⁸⁸

54

mKOκ

551

105000⁶⁹

2.08

0.61⁶⁹

58

TagRFP-T

557

81000⁴³

1.62

0.41⁴³

55

mRuby2

560

113000⁸⁶

2.12

0.38⁸⁶

58

mScarlet-I

569

102000³⁶

1.84

0.54³⁶

56

mCherry

587

72000⁶⁵

1.24

0.22⁶⁵

53

mKate2

588

62500⁷¹

1.15

0.40⁷¹

52