Table 2 Fluorescence lifetime data of mTurquoise2 as FRET donor in a tandem construct with the different FRET acceptors and corresponding FRET efficiency (Fig. 3).
Acceptor | n1 | τϕ (ns)2 | τM (ns)3 | Eτϕ (%)4 | EτM (%)4 |
|---|---|---|---|---|---|
— | 89 | 3.77 ± 0.01 | 4.01 ± 0.01 | — | — |
EGFP | 26 | 2.60 ± 0.01 | 3.21 ± 0.01 | 31 ± 0.26 | 20 ± 0.21 |
Clover | 18 | 2.59 ± 0.02 | 3.28 ± 0.02 | 31 ± 0.60 | 18 ± 0.62 |
mNeonGreen | 14 | 2.02 ± 0.01 | 2.70 ± 0.01 | 46 ± 0.33 | 33 ± 0.21 |
SYFP2 | 72 | 2.59 ± 0.02 | 3.16 ± 0.02 | 31 ± 0.57 | 21 ± 0.48 |
sREACh | 27 | 2.53 ± 0.01 | 3.13 ± 0.01 | 33 ± 0.26 | 22 ± 0.20 |
mOrange | 21 | 3.13 ± 0.03 | 3.63 ± 0.03 | 17 ± 0.92 | 9 ± 0.76 |
mOrange2 | 27 | 3.10 ± 0.04 | 3.61 ± 0.02 | 18 ± 0.99 | 10 ± 0.61 |
mKO2 | 17 | 2.59 ± 0.02 | 3.11 ± 0.02 | 31 ± 0.52 | 22 ± 0.44 |
mKOκ | 17 | 2.31 ± 0.01 | 2.77 ± 0.01 | 39 ± 0.42 | 31 ± 0.28 |
TagRFP-T | 20 | 2.70 ± 0.02 | 3.15 ± 0.02 | 28 ± 0.62 | 22 ± 0.44 |
mRuby2 | 17 | 2.63 ± 0.02 | 3.30 ± 0.02 | 30 ± 0.45 | 18 ± 0.45 |
mScarlet-I | 30 | 2.67 ± 0.02 | 3.21 ± 0.01 | 29 ± 0.64 | 15 ± 0.37 |
mCherry | 24 | 2.83 ± 0.02 | 3.26 ± 0.01 | 25 ± 0.42 | 19 ± 0.19 |
mKate2 | 22 | 2.74 ± 0.01 | 3.23 ± 0.02 | 27 ± 0.42 | 19 ± 0.40 |
