Table 3 FRET efficiencies of mTurquoise2 paired with the different acceptors, calculated from spectral imaging results (Fig. 4).

From: Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2

Acceptor

Number of cells

FRET efficiency (%)1

EGFP

37

44 ± 0.3

Clover

36

47 ± 0.7

mNeonGreen

46

59 ± 0.3

SYFP2

39

42 ± 0.7

mOrange

24

20 ± 1.5

mOrange2

22

21 ± 1.0

mKO2

34

35 ± 0.9

mKOκ

24

47 ± 0.2

TagRFP-T

50

17 ± 0.8

mRuby2

66

43 ± 1.2

mScarlet-I

47

34 ± 0.4

mCherry

28

32 ± 0.4

mKate2

27

28 ± 0.4

  1. 1E is the average FRET efficiency ± SEM
Back to article page