Table 1 Crystallization conditions.
From: Chimeric 14-3-3 proteins for unraveling interactions with intrinsically disordered partners
Chimera | 14-3-3σ Clu3 – HSPB6 phosphopeptide* | 14-3-3σ Clu3 – Gli1 phosphopeptide** | 14-3-3σ Clu3 – StARD1 phosphopeptide** | ||
Topology | bi-directional peptide swap | self-bound | mono-directional peptide swap | bi-directional peptide swap | |
Designation | pCH1℧ | pCH1X | pCH2 | pCH3 | |
Crystallization solution (reservoir) | 0.1 M MMT (malate-MES-Tris) pH 4, 25% PEG 1500 | 0.1 M Na-acetate pH 4.6, 20 mM CaCl2, 30% MPD | 0.1 M HEPES pH 7.5, 1 M Na-acetate, 50 mM CdSO4 | 0.1 M bis-Tris (pH 6.5), 2 M (NH4)2SO4 | 0.1 M bis-Tris-propane pH 6.5, 0.2 M (NH4)2SO4, 25% PEG 3350 |
Crystal handling | no cryosolution | no cryosolution (crystallization solution contained 30% MPD) | no cryosolution | cryosolution: 20 mM Tris pH 7.5, 0.1 M bis-Tris pH 6.5, 2.4 M (NH4)2SO4, 150 mM NaCl, 20% glycerol | no cryosolution |
Resolution, Å | 2.35 | 2.5–3.3 | 3.2 | 3.2 | 3.9 |
Protein conc. (mg/ml) | 23 | 23 (seeding) | 23 | 20.6 | 10.1 |
Temperature (°C) | 20 | 20 | 20 | 20 | 20 |
Growth time (days) | 8–12 | 1–4 | 3–6 | 8–14 | 7–10 |
