Figure 1
From: Identification and characterization of a novel Sso7d scaffold-based binder against Notch1
Screening for Sso7d variants against Notch1 ELR 11–15. (A) Crystal structure of Sso7d (PDB ID: 1SSO43) with randomized residues show in green sticks. Note: Image generated using Pymol. (B) Domain organization of Notch1. Green box highlights the ELR 11–15 domain of Notch1 chosen to screen for Sso7d-binders. (C) Schematics of Yeast Surface Display screen. After one round of magnetic screen with biotinylated-protein-bound magnetic beads (Dynabeads Biotin-binder), two rounds of selections were performed using Flow cytometry. Yeast isolated from magnetic screen, and two rounds of FAC sorting were double stained with 0.2 μM ELR 11–15 and anti-Myc antibody. Red circles depict enriched populations (D) Amino acid sequence of sso7d with randomized residues marked in red. (D’) Amino acid sequences of the candidate hits. Only the randomized residues are shown.
