Figure 3
From: Identification and characterization of a novel Sso7d scaffold-based binder against Notch1
N9 binds to ELR 11–13 and inhibits Notch ligand binding. (A) N9 inhibits Notch1/Jagged1 binding. ELR 11–15 coated wells were incubated with indicated concentrations of N9 and 100 nM Jagged1-Fc. Binding was measured using Protein-G HRP conjugate. (B) N9 binds to ELR 11–13 of Notch1. ELR 11–13 or a GST tagged control protein were incubated with N9 and pulled down using GST beads. (C) N9 inhibits Notch ligand binding. HEK 293 T cells transiently transfected with hNotch1 were preincubated with binders and 2 nM JAG1-Fc and DLL4 were added. Binding was detected using Anti-Fc APC antibody. Note that N9, but not N8 (scaffold control), decreases Notch ligand binding.
