Table 2 Seawater carbonate chemistry parameters in the algal cultures and percent carbon and nitrogen content of Isochrysis galbana cultured at 3 different pH conditions. Seawater total scale pH, temperature and total mean alkalinity (mean TA: 2156.13 μmol.kg−1 were used to calculate CO2 partial pressure (pCO2), aragonite and calcite saturation states (respectively ΩAr and ΩCa), for a salinity of 35.0 psu using the package seacarb for R. All values are expressed as mean ± SD.
Algal culture | Measured | Calculated | C:N ratio | ||||||
---|---|---|---|---|---|---|---|---|---|
T (°C) | pHT | AT (μmol/kg) | pCO2 (μatm) | ΩAr | ΩCa | mg C g−1 DW | mg N g−1 DW | C:N | |
Control | 27.64 ± 1.49a | 9.15 ± 0.39 | 2172.78 ± 26.80 | 21 ± 35a | 9.90 ± 1.55a | 14.41 ± 2.34a | 0.0367 | 0.0043 | 8.48a |
Medium pH | 27.42 ± 1.55a | 7.74 ± 0.13 | 2154.06 ± 39.47 | 903 ± 266b | 1.99 ± 0.62b | 2.99 ± 0.93b | 0.0464 | 0.0049 | 9.53a |
Low pH | 27.52 ± 1.49a | 7.39 ± 0.11 | 2141.54 ± 37.30 | 2146 ± 573c | 0.96 ± 0.24c | 1.45 ± 0.36c | 0.0490 | 0.0036 | 13.57b |