Figure 1

Morphology of HPG in culture. (A) Phase-contrast image of a typical HPG explant (around 100 cells/colony) cultured in vitro (10x) 24 h after seeding. (B) Phase-contrast image of HPG epithelial cells forming a monolayer (confluence of 90%) after a month of culture. (C) Picture showing intracellular keratin filaments (indicated by the arrow). A representative image is shown. (D) Phase-contrast images (10X) showing the morphology of HPG cells cultured in expansion medium (without calcium) and in expansion medium with 2 mM calcium for 24 h.