Figure 2 | Scientific Reports

Figure 2

From: Functional effects of proinflammatory factors present in Sjögren’s syndrome salivary microenvironment in an in vitro model of human salivary gland

Figure 2

Released amylase activity of HPG cells in culture. (A) Confocal images showing the differential interference contrast images (DIC), amylase immunoreactive (Alexa Fluor 488), the presence of intracellular actin filaments (Alexa Fluor 568 phalloidin), cell nucleous (DAPI) and a merged image in cultured HPG cells. (B) Bar graph showing the relative intracellular versus extracellular amylase activity in cells incubated with either expansion medium without or with 2 mM calcium. Data represent the relative mean ± SEM from eight independent experiments. (C) Bar graph showing amylase activity in the cell-free supernatant between cells cultures in expansion medium with 2 mM calcium or in enriched medium (expansion medium with 2 mM calcium and isoproterenol 10 µM). Data represent the relative mean ± SEM from eight independent experiments with respect to cells cultured in expansion medium plus calcium, which was considered 1. **p ≤ 0.01 by Wilcoxon signed-rank test.

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