Figure 4
From: Visualisation of a flexible modular structure of the ER folding-sensor enzyme UGGT
Cryo-EM structure of full-length UGGT. (a) 2D classes of UGGT particle subjected to single-particle cryo-EM analysis. (b) Negative-stain EM 3D reconstruction of UGGT with the Fab of monoclonal antibody directed against the Trx4 domain. The crystal structure of UGGTN fitted into the negative-stain EM map is coloured as in Fig. 1. The crystal structure of the CAT domain is shown on the same scale as a reference. The Fab fragment of the anti-Trx4 antibody is coloured black. (c) 3D classification of cryo-EM structures of UGGT. Each structure showed significant variations in the cryo-EM maps, but commonly shared a large central cavity.
