Table 1 Statistics of small RNA (sRNA) sequences from E (early lactation) and L (late lactation) libraries.

From: Screening of miRNA profiles and construction of regulation networks in early and late lactation of dairy goat mammary glands

Type

E library

L library

Number of total sRNA

Percentage of total (%)

Number of unique sRNA

Percentage of unique (%)

Number of total sRNA

Percentage of total (%)

Number of unique sRNA

Percentage of unique (%)

Raw reads

18,908,954

100

809,387

100

10,083,672

100

958,982

100

Junk reads

4,227

0.02

2,218

0.27

4,886

0.05

2,107

0.22

Repeat reads

38,984

0.21

5,927

0.73

25,990

0.26

5,693

0.59

3ADT &length filter*

1,197,580

6.33

566,670

70.01

1,851,160

18.36

713,666

74.42

Rfam

314,821

1.66

25.791

3.19

164,175

1.63

21,564

2.25

rRNA

163,022

0.86

10,825

0.06

91,082

0.90

9,750

0.10

tRNA

73,643

0.39

6,258

0.03

38,858

0.39

5,391

0.05

snoRNA

43,634

0.23

3,739

0.02

15,082

0.15

2,239

0.02

snRNA

7,751

0.04

1,355

0.01

2,455

0.02

1,063

0.01

other RNAs

26,771

0.14

3,614

0.02

16,698

0.17

3,121

0.03

mRNA

85,700

0.45

31,921

3.94

62,242

0.62

22,094

2.30

Valid reads

17,306,229

91.52

181,983

22.48

7,997,385

79.31

198,134

20.66

  1. *Reads lacking three ADTs or with lengths <18 nt or >26 nt were removed. 3ADT: reads with null 3′ adapter. snRNA: small nuclear RNA. snoRNA: small nucleolar RNA. tRNA, rRNA snRNA, snoRNA and other RNAs were obtained by blasting sRNAs against Rfam database.
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