Figure 1

The closed neural tube developed two dorsal neuroepithelial protrusions in G16 Trachemys scripta embryo. Hematoxylin and eosin stained transverse paraffin sections demonstrated a change in the morphology of the neuroepithelial cells forming the roof plate. (A) At G15+, there was a uniform and rounded roof plate formed by the neuroepithelial cells. The morphology was similar to stage TK15 in the soft-shelled turtle Pelodiscus sinensis and stage HH28 in chick48. (B) At G16, the roof plate had two ‘folds’ of neuroepithelial cells protruding dorsally (circled). (C,D) The protruding roof plate morphology was seen also at G17 (C) and G18 (D). Scale bar approx. 100 μm (A–D). c, cartilage; drg, dorsal root ganglion; n, notochord; s, somite.