Figure 5

Melanoblast development coincided with the second phase cell emigration. (A) Pigment containing cells (black) differentiated from the migratory cells derived from G16 trunk neural tube during a 14 day cell culture. (B,C) Anti-Mitf antibody stained melanoblasts in the dorsal neural tube, the CSA, and the surface ectoderm at G15+ and G16. (D) c-Kit positive melanocytes were visible in the CSA above the neural tube and in the surface ectoderm at G21. Scale bars approx. 30 μm (A), 100 μm (B–D).