Figure 3

hSef-b suppresses tumor growth in vivo. (A) Average volume of tumors transfected with control empty vector (N = 14) or with hSef-b construct (N = 12) by TUS. Tumor volume was measured at weekly intervals during the 3 rounds of DNA injections. (B) Average tumor weight at the end of the experiments (***p ≤ 0.0001). (C) Images of representative tumors. Bar: 2.5 mm. (D) RT-PCR analysis for the detection of hSef-b transcript in total RNA extracted from the resected tumors. Amplification was carried out with hSef-b specific primers.