Figure 2
Effects of different operational parameters on the performance of the on-chip assay. (a) concentration of thrombin (0.29 mM S-2238), (b) concentration of S-2238 (0.2 U/mL thrombin), (c) reaction time of thrombin and S-2238 (0.2 U/mL thrombin, 0.31 mM S-2238), (d) incubation time of thrombin and QSYQ sample (0.2 U/mL thrombin, 0.31 mM S-2238), (e) concentration of ACE (0.36 mM FAPGG), (f) concentration of FAPGG (0.027 U/mL ACE), (g) incubation time of ACE and QSYQ sample (0.027 U/mL ACE, 0.72 mM FAPGG). Assays were carried out in triplicate except (e) and (f), and results were expressed as mean ± S.D. Assays for (e) and (f) were performed once, and the absorbance was measured every three minutes. For ACE assay, Δabsorbance was the absorbance decrease between 0 min and 30 min after the addition of FAPGG at 340 nm.
