Figure 1
From: Adaptation trajectories during adhesion and spreading affect future cell states
Cell spreading dynamics is very different for PAAm and protein gels (a) hMSC spreading at time points that illustrate the route of spreading on the six gel types. Representative cells were selected for each time point. Scale bar 50 µm. (b) Actin structure during the different trajectories of spreading showing the radial and transverse fibres after 1 h on the stiff PAAm gels and the onset of spreading with the formation of protrusions after 5 h on the protein gels. Scale bar 50 µm. (c) Quantification of cell area and (d) perimeter over time, showing the different spreading trajectories on PAAm and protein gels. Mean ± SEM from three replicates (mean from two for medium PAAm) with over 50 cells analysed per sample per replicate, ANOVA oneway analysis followed by Tukey post hoc correction per timepoint is shown in Tables S1 and S2.
