Figure 3
From: Adaptation trajectories during adhesion and spreading affect future cell states
Gel deformation and cell spreading over time: (a) Round (left) and spread (right) hMSCs stained for actin (red) on labelled collagen and fibrin gels (green). Both round and spread cells can deform the matrix. All scale bars represent 50 µm. (b) Gel deformation was quantitatively measured by bead displacement. The cells on PAAm gels do barely deform the matrix, whereas the cells on collagen and fibrin can deform the gel over long distances. (c) Average displacement of beads within 150 µm of cells on PAAm-col, PAAm-fb, collagen or fibrin. (d) Comparison between onset of deformation and spreading. On collagen, all cells first deform the matrix before they started to spread (top left part). On fibrin, approximately half of the cells first deformed the matrix, the others only deformed the matrix after spreading (bottom right part).
