Figure 4
From: Adaptation trajectories during adhesion and spreading affect future cell states
Differences in dynamic gene expression programs associate with observed cell spreading behaviour and highlights underlying mechanisms (a) Venn diagrams summarising the magnitude of the overlap in top 25 upregulated (left) and downregulated (right) genes per substrate after 24 h showing the divergence in RNA-expression profiles. (b) Venn diagrams summarising the magnitude of the overlap in top 25 upregulated (left) and downregulated (right) genes per substrate after 1 h at the protein gels and 5 h at the PAAm gels indicating that the difference in number of differentially expressed genes after 1 h is not simply delayed on PAAm gels compared to the protein gels. (c) Heat map representation of the Z-scores of gene expression levels clustered by K-means clustering (d) Principal Component 4 can distinguish the PAAm substrates from the protein gels (e) Average relative expression levels from genes in cluster 6 show differences in gene expression on PAAm and protein gels (f) Selection of significantly overrepresented GO terms (p < 0.001) per cluster.
