Figure 3
From: Deoxyribonuclease 1 reduces pathogenic effects of cigarette smoke exposure in the lung
Deoxyribonculease 1 disrupts macrophage and neutrophil extracellular trap formation. The addition of deoxyribonuclease (DNase) 1 reduces the expression of cigarette smoke-induced METs. MET expression in vitro (A,B): (A) CSE-induced human BAL METs (chromatin/MMP9/12), (n = 20), and (B) CSE-induced human BAL METs (chromatin/H3Cit), (n = 14). MET expression in vivo in murine lung (C,D): (C) BAL METs (n = 10), and (D) lung tissue METs (n = 10). The addition of DNase 1 reduces the expression of cigarette smoke-induced NETs (E,F): (E) CSE-induced human blood NETs (n = 8), and (F) NETs as characterised using flow cytometry (n = 8). NET expression in vivo in mice: (G) lung tissue NETs (n = 10). Results are shown as smoke exposure (in vitro CSE or in vivo cigarette smoke exposure) and the effect of the addition of DNase 1 on this exposure.
