Figure 7

Heatmap showing the resistome profile in the GI tract of feedlot steers. Steers were fed Monensin and Tylosin as feed additives (AB) or control animals were not given any treatments (NA). Samples were collected from rumen, colon, and cecum and DNA was subjected to metagenome sequencing using Illumina platform. The raw files were de novo assembled and antibiotic resistance genes were identified searching the assembled contigs against a local copy of ResFinder database using BLAST. The yellow color represents absence of the antibiotic resistance genes and orange color indicates the presence. The macrolide resistance genes erm(F) and erm(G) were detected only in rumen of 3 AB steers.