Figure 4

(a) Fluorescence signal trace collected from viruses in the three intersecting channels with each channel containing one virus strain labeled with a green (Cy3) dye, and one virus strain labeled with red (Cy5) dye, and simultaneously excited with wavelengths λ = 525 nm and λ = 633 nm. They are subsequently identified uniquely by determining the number of spots in each individual peak. (b) Peak patterns observed for each type of virus strain with the green excitation (λ = 525 nm) generating N = 10, 7 and 5 spots and red excitation (λ = 633 nm) generating N = 8, 6 and 4 spots at channels 1, 2 and 3 respectively.