Figure 2
Repetitive corticoreticular activation induces a GluN2C-NMDAR-dependent increase in nRt excitability. (a) Left, time course of corticoreticular EPSP slope (analysed for % peak amplitude of the differential of the voltage signal, dV/dt) and of the membrane potential (Vm) during baseline and after repetitive photostimulation (n = 6). Right: top, example response elicited by the train stimulation (10 Hz), which was repeated 30 times every 30 s; middle, examples of EPSPs during baseline and after induction, showing the increase in low-threshold firing, which is quantified in the bar graph (n = 9); bottom, example of average EPSPs and corresponding differential traces during baseline (1) and at the end of the recording (2). (b) Same representation as in (a) for experiments conducted in the presence of PPDA (500 nM, n = 8). (c) Left, time course of corticoreticular EPSCs recorded at −70 mV and of holding current (Ihold) during baseline and after repetitive photostimulation (n = 6), indicating no change in AMPAR-mediated transmission. Right, examples of responses elicited during the train stimulation (performed in current-clamp) and average EPSCs recorded during baseline (1) and at the end of the recording (2). *p < 0.05, **p < 0.01, two-sided paired t test.
