Figure 3
Expression of human TF in glioma cell lines. (a) Human TF copy number of glioma cell lines was determined using quantitative real-time RT-PCR. Human TF copy number in U87MG, U251MG, U118MG, LN18, LN229, U87MG/Luc and U87MG/Luc/TF was 3.90 ± 0.94 copies/cell, 6.57 ± 2.14 copies/cell, 7.16 ± 1.08 copies/cell, 200.36 ± 3.58 copies/cell, 6.44 ± 4.04 copies/cell, 2.77 ± 1.28 copies/cell and 44.13 ± 1.64 copies/cell, respectively. The experiments were repeated three times, and the data are shown as the means ± standard deviation (SD). (b) Expression of human TF protein on glioma cell lines was evaluated using flow cytometry. Relative expression of human TF protein normalized to the negative control in U87MG, U251MG, U118MG, LN18, LN229, U87MG/Luc and U87MG/Luc/TF was 0.98, 3.73, 2.44, 85.96, 1.82, 1.16 and 40.83, respectively.
