Figure 8
Autoradiography. The upper, middle and lower panels show HE staining, TF immunostaining and autoradiography, respectively. The radioactivity derived from 111In-labelled antibodies was observed in the intracranial tumour tissues in both the anti-TF 1849 IgG-administered (left panels) and control IgG-administered mice (right panels), whereas the radioactivity in normal brain tissues was barely detectable in both groups. Autoradiography confirmed that 111In-labelled anti-TF 1849 IgG (lower left panel) more efficiently accumulated in the intracranial tumours overexpressing TF compared with 111In-labelled control IgG (lower right panel). Anti-TF 1849 IgGs were heterogeneously distributed in the tumour tissues (lower left panel), whereas TF immunostaining showed that TF was homogeneously expressed in the tumours (middle left panel). Scale bar, 4 mm.
