Figure 4
Phenotypes of Cdk5 knockdown mice. (A) Cdk5 knockdown mice showing lightened hair color compared to the wild-type mice. (B) (a-b) Hematoxylin staining of skin sections of Cdk5 knockdown mice compared with the wild-type mice. sb, stratum basale; sg, stratum granulosum; ss, stratum spinosum. (c-d) Fontana-Masson staining of skin sections of Cdk5 knockdown mice compared with the wild-type mice. (C) Spectrophotometric measurement for alkali-soluble melanins in the hair of Cdk5 knockdown mice compared to the wild-type mice. Bars represent the mean ± standard error (n = 3). ***P < 0.001. (D) (a) comparison of the ESR signal in the hair between wild-type mice and Cdk5 knockdown mice. b: The ESR signal of the hair in wild-type mice. (c) The ESR signal of the hair in Cdk5 knockdown mice. The following analytical conditions were used: a microwave frequency of 9050.999 MHz, field of 323 ± 5 mT, modulation amplitude of 0.1 mT, microwave power of 0.998 mW and frequency of 9050.999 MHz, and time constant of 0.03 s. The g value was 1.99863.
