Figure 6
Analysis of K10 protein expression in the skin of Cdk5 knockdown mice. (A) Immunohistochemical analysis of K10 protein expression in the skin of Cdk5 knockdown mice. (B) Western blot analysis of K10 protein expression in the skin of Cdk5 knockdown mice compared with wild-type mice. (C) The abundance of the K10 protein was quantified using Image-Pro Plus software and normalized relative to the abundance of the β-actin protein. (D) Co-IP experiments were performed using an anti-Cdk5 antibody followed by Western blot analysis using an anti-K10 antibody and anti-Cdk5 antibody. (E) Reciprocal co-IP experiments using an anti-K10 antibody were performed followed by a Western blot analysis using an anti-Cdk5 antibody and anti-K10 antibody. Bars represent the mean ± standard error (n = 3). ***P < 0.001.
