Figure 3
From: Evidence for long-term sensitization of the bowel in patients with post-infectious-IBS
Rectal biopsy supernatants from PI-IBS patients directly activate and increase the mechanosensitivity of serosal lumbar splanchnic afferents. A and B are representative examples of supernatant-induced single serosal fiber response profiles over 12 minutes ring application (indicated as a horizontal bar), respectively from a HV and a PI-IBS biopsy. From top to bottom as successively depicted the firing rate (0.60 s−1), the wavemarked neurogram (mV), and the raw neurogram (mV). Averaged peak change in activity during HV (n = 8) or PI-IBS (n = 8) supernatant incubation are summarized as histograms in (E). Data are presented as mean ± SEM and significant difference indicated (Unpaired t-test, *p < 0.05). Mechanical probes with von Frey hair (600 mg) were performed prior to and after supernatant application. (C and D) are representative responses to single 3 seconds von Frey hair probing of the corresponding receptive field successively before (left panels) and after (right panels) supernatant incubation. Top panels are instantaneous frequency (Hz), middle and bottom panels are as previously described. Changes in mechanosensitivity (HV: n = 8 and PI-IBS: n = 8) are summarized as histograms in (F). Data are presented as mean ± SEM and significant difference indicated (Paired t-test, *p < 0.05). Hz = hertz, HV = healthy volunteer, PI-IBS = post-infectious irritable bowel syndrome.
