Figure 1 | Scientific Reports

Figure 1

From: Cytokine-induced autophagy promotes long-term VCAM-1 but not ICAM-1 expression by degrading late-phase IκBα

Figure 1

Pro-inflammatory cytokines induce autophagy which facilitates Jurkat cells adhesion to HUVECs. (A,B) HUVECs were treated with 10 ng/mL IL-1β (A) or 10 ng/mL TNFα (B) for indicated time. LC3B was analyzed by western blotting. (C,D) HUVECs were treated with 100 nmol/L rapamycin, 10 ng/mL IL-1β, or 10 ng/mL TNFα for 6 h. LC3B and Beclin-1 (C) and phospho-S6K and S6K (D) were analyzed by western blotting. (E) HUVECs were treated with 10 ng/mL IL-1β or 10 ng/mL TNFα with or without 5 mmol/L 3-MA for 24 h. Leukotracker-labeled Jurkat cells were then added onto HUVECs for 1 h. Adhesion of Jurkat cells was determined by the fluorescence at 480 nm/520 nm. Each bar denotes mean ± SD of three independent experiments. *Indicates P < 0.05 vs. IL-1β or TNFα alone.

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