Figure 7 | Scientific Reports

Figure 7

From: Cytokine-induced autophagy promotes long-term VCAM-1 but not ICAM-1 expression by degrading late-phase IκBα

Figure 7

3-MA inhibits cytokines-induced IP-10 expression. (A) HUVECs were pretreated with or without 5 mmol/L 3-MA for 30 min followed by 10 ng/mL IL-1β or 10 ng/mL TNFα for 24 h. Conditioned medium was collected and cytokines in the medium were analyzed by an antibody array. Upper panel shows representative dot blots of 80 cytokines/chemokines. Arrows indicate dots of IP-10. The lower panel shows the place of each cytokine within the array. (B) Densitometric analysis of IP-10 in the dot blots (n = 2). (C) IP-10 in the medium was analyzed by ELISA. Each bar denotes mean ± SD of three independent experiments. *Indicates P < 0.05.

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