Table 1 Primer sequences and function used in this study.
From: CCK reduces the food intake mainly through CCK1R in Siberian sturgeon (Acipenser baerii Brandt)
Primer name | Primer sequence (5′ to 3′) | Applications |
|---|---|---|
cck-F | GCTATGAACAGTGGAATC | cck-cloning |
cck-R | GGCTACCCACTGTATTGT | cck-cloning |
cck-F1 | TGAAAACGGCAGAGGGAAACAT | cck-3′ RACE outer |
cck-F2 | TTTAGAGCCAGACAGTTGGGAGA | cck-3′ RACE inner |
cck-R1 | CGTCCAAAGTCCATCCAGCCCAT | cck-5′ RACE outer |
cck-R2 | GTCCAAGGTTCGCTCTTTGCTCC | cck-5′ RACE inner |
cck-qF | GAGGGTAGTCCTGTAGCATCTGA | cck-qPCR |
cck-qR | TTCTACCAGACGAGCCTTTCC | cck-qPCR |
ucn3-qF | CAGGGGAGGAGAGAGAAAAAA | ucn3-qPCR |
ucn3-qR | CTGAGACATTAGGCGAGCGT | ucn3-qPCR |
nucb2-qF | TGGAGACAGACCAGCATTTCAG | nucb2-qPCR |
nucb2-qR | GGCTCCGTAACCTGTTCACTTC | nucb2-qPCR |
cart-qF | CGACTGTGGTTGAGAGCCG | cart-qPCR |
cart-qR | GACAGTCACACAACTTGCCGAT | cart-qPCR |
apelin-qF | CAGACACGCTGTTTTACACCAC | apelin-qPCR |
apelin-qR | GCACAGCATGGACACCAAGAT | apelin-qPCR |
pyy-qF | AGGCAGAGGTATGGCAAGCG | pyy-qPCR |
pyy-qR | GGAGGGTCAGGAGACGGGAT | pyy-qPCR |
npy-qF | GCTGGCTACCGTGGCTTTC | npy-qPCR |
npy-qR | GACTGGACCTCTTCCCATACCT | npy-qPCR |
β-actin-qF | GTTGGTATGGGACAGAAGGACA | β-actin-qPCR |
β-actin-qR | CCAGTTGGTAACAATGCCGT | β-actin-qPCR |
gapdh-qF | CATTTGATGTTGGCTGGGT | gapdh-qPCR |
gapdh-qR | CTTTCTGGGAAGGTGGAGGT | gapdh-qPCR |
