Figure 2
From: The autophagy scaffold protein ALFY is critical for the granulocytic differentiation of AML cells
ALFY is down-regulated in primary AML and upregulated during normal neutrophil differentiation. (a) RNA was isolated from 95 patients with primary AML of FAB subtype M0-M4 and qPCR analysis of ALFY mRNA was performed using the TaqMan Low-Density Array (TLDA). Raw Ct values were normalized to HMBS and ABL1 (−∆Ct). −∆Ct values of patient samples harbouring different chromosomal aberrations are shown and compared to the −∆Ct values of granulocytes from healthy donors (Gran.). CK = complex karyotype, NK = normal karyotype. Statistical test: Kruskall-Wallis test followed by corrected Dunn’s post hoc test for multiple comparison. *P < 0.05, **P ≤ 0.01, ***P ≤ 0.001. (b) CD34+ cells from three different donors (1–3) were in vitro differentiated towards granulocytes using G-CSF. ALFY transcript levels were measured at day 0 (d0), day 3 (d3) and day 6 (d6) using qPCR and normalized to HMBS. Friedman test followed by corrected Dunn’s post hoc test for multiple comparison was applied. * p < 0.05. (c) The panel displays the results obtained from the “normal haematopoiesis with AMLs” dataset contained in the bloodspot database. Please refer to the website for details on the dataset characteristics. HSC: hematopoietic stem cells, MPP: multipotent progenitors, CMP: common myeloid progenitor, GMP: granulocyte monocyte progenitor, PM: promyelocyte, MM: metamyelocyte, BC: band cell, PMN: polymorph nuclear cells. Kruskall-Wallis test followed by corrected Dunn’s post hoc test for multiple comparison was applied. **** p ≤ 0.0001.
