Figure 1
From: Revealing hidden complexities of genomic rearrangements generated with Cas9
Overview of the 155.3 kb rearrangement region targeting Nox4. (A) The position of the gRNAs 5a, 5b, 3a and 3b are shown as black arrowheads. Four FISH probes across the Nox4 region are illustrated, two are external to the gRNA cut side and two are within the Nox4 gene. Primers for junction PCRs are depicted as arrowheads with the forward primers (F) marked green/blue and the reverse primers (R) being red/purple. (B) Schematic showing the different types of rearrangements that could be present within a founder animal, the corresponding PCRs used to identify them and the colour and order of the FISH probes.
